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The web-server iSMP-Grey was establish for identifying uncharacterized proteins as secretory protiens of malaria parasite or not according to their evolutionary information in form of position specific scoring matrix (PSSM). The anticipated overall success rate is about 94%, which was derived via rigorous jackknife tests on a benchmark dataset.
To obtain the predicted result with the anticipated success rate, the entire sequence of the query protein rather than its fragment should be used as an input. A sequence with less than 50 amino acid residues is generally deemed as a fragment. Also, a sequence with more than 10 consecutive character of "X" is not a valid input because containing too many unknown amino acids.